Transcription of the tyrosinase gene family in an Atlantic salmon leukocyte cell line (SHK-1) is influenced by temperature, but not by virus infection or bacterin stimulation

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• 作者：Hilde A.S. Larsen^a ; Lars Austb&oslash ; ^b ; Melanie Kö ; nig^b ; Henning S&oslash ; rum^c ; Espen Rimstad^c ; Erling O. Koppang^a ; ^{erling.o.koppang@nvh.no}
• 关键词：Melanin ; Melanogenesis ; Melanomacrophage ; SHK-1 cells
• 刊名：Developmental and Comparative Immunology
• 出版年：2013
• 出版时间：September, 2013
• 年：2013
• 卷：41
• 期：1
• 页码：50-58
• 全文大小：1744 K

文摘

The present study was performed to address putative links between the immune and pigmentary systems. A pigment-producing leukocyte-like cell-line (SHK-1 cells) of Atlantic salmon (Salmo salar L.) was exposed to different temperatures, poly I:C, bacterin or infected with virus (infectious pancreatic necrosis virus or infectious salmon anaemia virus). The effect of this stimulation regarding the transcription-pattern of the tyrosinase gene family (melanin genes) and the immune-related genes MHC class II and IFN-1 was analysed using real-time RT-qPCR. At 10 ¡ãC cultivation, tyrosinase and dopachrome tautomerase remained unregulated. At 15 ¡ãC, a moderate up-regulation was induced, while at 20 ¡ãC, these genes were up-regulated in an exponential manner over time. Temperature did not affect the transcription of the immune-related genes. Virus infections, poly I:C or bacterin had no influence on the transcription of the melanogenesis-related genes, but triggered the immune-related genes. Our findings revealed no connections between the pigmentary and immune systems, but demonstrated a hereto undiscovered temperature-effect on the tyrosinase gene family.