Effect of heparin on the biological properties and molecular signature of human mesenchymal stem cells
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- 作者：Ling Linga ; 1 ; Emily T. Camillerib ; 1 ; Torben Hellediea ; Rebekah M. Samsonraja ; b ; Drew M. Titmarsha ; Ren Jie Chuaa ; Oliver Dreesena ; Christian Dombrowskia ; David A. Ridera ; Mario Galindoc ; d ; Ian Leee ; Wanjin Honge ; James H. Huif ; Victor Nurcombea ; Andre J. van Wijnenb ; vanwijnen.andre@mayo.edu" class="auth_mail" title="E-mail the corresponding author ; Simon M. Coola ; f ; simon.cool@imb.a-star.edu.sg" class="auth_mail" title="E-mail the corresponding author
- 关键词：hMSCs ; human bone marrow-derived mesenchymal stem cells ; RNA-seq ; high throughput next generation RNA sequencing ; FGF ; fibroblast growth factor ; HBD ; heparin-binding domain ; VEGFs ; vascular endothelial factors ; UFH ; unfractionated heparin ; LMWH ; low-molecular-weight heparin ; hESC ; human embryonic stem cell ; HS ; heparan sulfate ; ECM ; extracellular matrix ; FCS ; fetal calf serum ; BMMNCs ; bone marrow mononuclear cells ; BrdU ; 5-bromo-2-deoxyuridine ; SVD ; singular value decomposition ; ALP ; alkaline ph
- 刊名：Gene
- 出版年：2016
- 出版时间：15 January 2016
- 年：2016
- 卷：576
- 期：1
- 页码：292-303
- 全文大小：1783 K
文摘
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Heparin is a common cell culture adjuvant for the expansion of human stem cells.
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Heparin affects multiple cell signaling components that can alter human mesenchymal stem cell responsiveness.
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Heparin exerts donor-dependent effects on gene expression and proliferation of human mesenchymal stem cells.
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Heparin is contra-indicated as a culture supplement for the expansion and maintenance of naïve human mesenchymal stem cells.