Identification and in silico characterization of two novel genes encoding peptidases S8 found by functional screening in a metagenomic library of Yucatán underground water
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- 作者：Max M. Apolinar&ndash ; Herná ; ndeza ; Yuri J. Peñ ; a&ndash ; Ramí ; rezb ; Ernesto Pé ; rez-Ruedac ; 1 ; Blondy B. Canto-Canché ; a ; Cé ; sar De los Santos-Brionesa ; Aileen O'Connor-Sá ; ncheza ; aileen@cicy.mx" class="auth_mail" title="E-mail the corresponding author
- 关键词：Culture-independent analysis ; Enzymes ; Metagenomics ; Proteases ; Serine proteases ; Yucatá ; n aquifer
- 刊名：Gene
- 出版年：2016
- 出版时间：15 November 2016
- 年：2016
- 卷：593
- 期：1
- 页码：154-161
- 全文大小：1308 K
文摘
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A fosmid metagenomic library of tropical underground water was constructed.
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Two novel protease S8A genes were identified by functional screening.
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An extensive in silico characterization showed that their ORFs encode complex zymogens.
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Independent sub-cloning of these two genes showed that both encode functional proteases.
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Modeling suggests that they encode subtilases which hydrolyze a broad range of peptide bonds.