Live imaging of muscles in Drosophila metamorphosis: Towards high-throughput gene identification and function analysis
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- 作者：Wee Choo Puah serenity.pwc@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Martin Wasser ; mwasser07@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Muscle ; Time-lapse microscopy ; Metamorphosis ; Drosophila ; Atrophy
- 刊名：Methods
- 出版年：2016
- 出版时间：1 March 2016
- 年：2016
- 卷：96
- 期：Complete
- 页码：103-117
- 全文大小：4150 K
文摘
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Abdominal muscles in Drosophila metamorphosis are a model for muscle wasting.
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Time lapse microscopy provides new insights into atrophy and histolysis of muscles.
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Non-invasive live imaging achieves higher throughput than analysis of fixed tissues.
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Targeted RNAi can manipulate muscle size and programmed cell death.
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Better understanding of protein localization using new fluorescent reporters.