Validated methods for determination of neurotransmitters and metabolites in rodent brain tissue and extracellular fluid by reversed phase UHPLC-MS/MS
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- 作者：Marianne Skov-Skov Bergha ; Marianne.Skov.Skov.Bergh@fhi.no" class="auth_mail" title="E-mail the corresponding author ; Inger Lise Bogena ; Elsa Lundanesb ; Å ; se Marit Leere Ø ; iestada
- 关键词：5-HIAA ; 5-hydroxyindoleacetic acid ; 5-HT ; Serotonin ; 3-MT ; 3-methoxytyramine ; ACh ; Acetylcholine ; ACN ; Acetonitrile ; DA ; Dopamine ; DOPAC ; 3 ; 4-dihydroxyphenylacetic acid ; ECF ; Extracellular fluid ; ESI ; Electrospray ionization ; FA ; Formic acid ; GABA ; γ-aminobutyric acid ; Glu ; Glutamic acid ; HCl ; Hydrochloride ; HVA ; homovanillic acid ; IS ; Internal standard ; LC ; Liquid chromatography ; LLOQ ; Lower limit of quantification ; LOD ; Limit of detection ; LR ; Linear range ; ME ; Matrix effects ; MeOH ; Methanol
- 刊名：Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences
- 出版年：2016
- 出版时间：15 August 2016
- 年：2016
- 卷：1028
- 期：Complete
- 页码：120-129
- 全文大小：1170 K
文摘
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Fast and sensitive UHPLC–MS/MS-method for determination of neurotransmitters.
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Validated method for analysis of both brain extracellular fluid and brain tissue.
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Surrogate analytes used in validation of endogenous compounds.
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One of few developed neurotransmitter methods that include 3-MT.