Differential sensitivity of metabolically competent and non-competent HepaRG cells to apoptosis induced by diclofenac combined or not with TNF-α
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- 作者：Houssein Al-Attrachea ; b ; d ; Ahmad Sharaneka ; b ; Audrey Burbana ; b ; Matthew Burbanka ; b ; Thomas Gicquela ; b ; c ; Ziad Abdel-Razzakd ; Christiane Guguen-Guillouzoa ; b ; Isabelle Morela ; b ; c ; André ; Guillouzoa ; b ; Andre.Guillouzo@univ-rennes1.fr" class="auth_mail" title="E-mail the corresponding author
- 关键词：ABT ; 1-aminobenzotriazole ; Ac-DEVD-AMC ; N-acetyl-Asp-Glu-Val-Asp-7-amido-4-methylcoumarin ; AC-IETD-AMC ; Ac-Ile-Glu-Thr-Asp-7-amino-4-methylcoumarin ; AC-LEHD-AMC ; Ac-Leu-Glu-His-Asp-7-Amino-4-methylcoumarin ; BSEP ; bile salt export pump ; CDF ; 5 (and 6)-carboxy-2&prime ; 7&prime ; -dichlorofluorescein (CDF) ; CRP ; C-reactive protein ; CYP ; cytochrome P450 ; DCF ; diclofenac ; DMSO ; dimethyl sulfoxide ; ER ; endoplasmic reticulum ; H2-DCFDA ; 2&prime ; 7&prime ; -dichlorodihydrofluorescein ; HO1 ; heme oxygenase 1
- 刊名：Toxicology Letters
- 出版年：2016
- 出版时间：6 September 2016
- 年：2016
- 卷：258
- 期：Complete
- 页码：71-86
- 全文大小：3960 K
文摘
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HepaRG hepatocytes are less sensitive to DCF than HepG2 cells and undifferentiated HepaRG cells.
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The lower sensitivity of HepaRG hepatocytes is related to their high detoxifying capacity.
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Inhibition of glutathione transferases results in increased DCF cytotoxicity.
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TNF-α potentiation of DCF cytotoxicity is not observed in undifferentiated HepaRG cells.
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DCF-induced cholestasis typified by bile canaliculi dilatation is not aggravated by TNF-α.