Novel helical assembly in arginine methyltransferase 8
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- 作者：Sachiko Toma-Fukai1 ; Jun-Dal Kim2 ; Kyung-Eui Park3 ; Naoyuki Kuwabara4 ; Nobutaka Shimizu5 ; Elena Krayukhina6 ; 7 ; Susumu Uchiyama6 ; Akiyoshi Fukamizu2 ; 3 ; Toshiyuki Shimizu1 ; shimizu@mol.f.u-tokyo.ac.jp" class="auth_mail" title="E-mail the corresponding author
- 关键词：PRMT8 ; Protein arginine methyltransferase 8 ; SEC ; size exclusion chromatography ; SAXS ; small angle X-ray scattering ; MALS ; multi-angle light scattering ; AUC ; analytical ultracentrifugation ; SAH ; S-adenosyl homocysteine ; BiFC ; bimolecular-fluorescence complementation ; Sc ; Saccharomyces cerevisiae ; PBS ; phosphate-buffered saline ; CBB ; Coomassie Brilliant Blue ; HEK ; human embryonic kidney
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：27 March 2016
- 年：2016
- 卷：428
- 期：6
- 页码：1197-1208
- 全文大小：1269 K
文摘
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PRMT8 is a type I protein arginine methyltransferase.
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10">The crystal structure of PRMT8 exhibits a novel helical assembly.
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15">The oligomerization is necessary for proper function of this enzyme.
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The oligomerization state is required for cellular localization.