Utilization of unmodified gold nanoparticles for label-free detection of mercury (II): Insight into rational design of mercury-specific oligonucleotides
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- 作者：Abdul Ghaffar Memona ; b ; Xiaohong Zhoua ; xhzhou@tsinghua.edu.cn" class="auth_mail" title="E-mail the corresponding author ; 26877421@qq.com" class="auth_mail" title="E-mail the corresponding author ; Jinchuan Liua ; Ruoyu Wanga ; Lanhua Liua ; Bofan Yua ; Miao Hea ; hemiao@tsinghua.edu.cn" class="auth_mail" title="E-mail the corresponding author ; Hanchang Shia
- 关键词：AuNPs ; gold nanoparticles ; DLS ; dynamic light scattering ; HR-TEM ; high resolution transmission electron microscopy ; LSPR ; local surface plasmon resonance ; MBs ; magnetic beads ; MOPS ; 3-morpholinopropane-1-sulfonic acid ; MSO ; mercury-specific oligonucleotides ; PDI ; poly disparity index ; RT ; room temperature ; TEM ; transmission electron microscopy ; T&ndash ; Hg2+&ndash ; T ; thiamine-mercury-thiamine
- 刊名：Journal of Hazardous Materials
- 出版年：2017
- 出版时间：5 January 2017
- 年：2017
- 卷：321
- 期：Complete
- 页码：417-423
- 全文大小：1041 K
文摘
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A rational design of Hg2+-specific ssDNA (MSO) was proposed for the colorimetric biosensing system using unmodified AuNPs as indicators.
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The leftover bases of MSO as a result of T–T mismatch can absorb on the AuNPs.
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The DLS proved the size increase of MSO:DNA conjugate due to Hg2+ addition.
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An improved LOD value by ∼20-fold was achieved by cutting the leftover bases.