To investigate the influence of Nux vomica and Calendula officinalis on HB-EGF-like growth factor gene expression in KATO-III cells under the stimulation of H. pylori strain N6 using real-time PCR with and without addition of Nux vomica and Calendula officinalis as a 10c or 12c potency.
Baseline expression and stimulation were similar to previous experiments, addition of Nux vomica 10c and Calendula officinalis 10c in a 43 % ethanolic solution led to a significant reduction of H. pylori induced increase in gene expression of HB-EGF (reduced to 53.12 ± 0.95 % and 75.32 ± 1.16 % vs. control; p < 0.05), respectively. Nux vomica 12c reduced HB-EGF gene expression even in dilutions beyond Avogadro’s number (55.77 ± 1.09 % ; p < 0.05). Nux vomica 12c in a 21.5 % ethanol showed a smaller effect (71.80 ± 3.91 % , p < 0.05). This effect was only be observed when the drugs were primarily prepared in ethanol, not in aqueous solutions. The data suggest that both drugs prepared in ethanolic solution are potent inhibitors of H. pylori induced gene expression.