Cellular electron cryo tomography and in situ sub-volume averaging reveal the context of microtubule-based processes

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• 作者：Michael Grange ; Daven Vasishtan ; Kay Grü ; newald ; ^{kay@strubi.ox.ac.uk}
• 关键词：Electron cryo-tomography ; Cellular architecture ; Sub-volume averaging ; Microtubules ; Cytoskeleton ; Dynein ; Adenovirus ; Electron cryo-microscopy ; Virus trafficking ; Viral entry ; Retrograde transport ; Endocytosis ; in situ structure determination
• 刊名：Journal of Structural Biology
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：197
• 期：2
• 页码：181-190
• 全文大小：4103 K
• 卷排序：197

文摘

Electron cryo-tomography (cryoET) is currently the only technique that allows the direct observation of proteins in their native cellular environment. Sub-volume averaging of electron tomograms offers a route to increase the signal-to-noise of repetitive biological structures, such improving the information content and interpretability of tomograms. We discuss the potential for sub-volume averaging in highlighting and investigating specific processes in situ, focusing on microtubule structure and viral infection. We show that (i) in situ sub-volume averaging from single tomograms can guide and complement segmentation of biological features, (ii) the in situ determination of the structure of individual viruses is possible as they infect a cell, and (iii) novel, transient processes can be imaged with high levels of detail.