Identification of proteasome subunit beta type 2 associated with deltamethrin detoxification in Drosophila Kc cells by cDNA microarray analysis and bioassay analyses
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- 作者：Junli Hua ; 1 ; Dongxu Jiaoa ; 1 ; Qin Xub ; Xiaoli Yinga ; Wei Liua ; Qingping Chia ; Yuting Yea ; Xueyu Lia ; Luogen Chenga ; chengluogen@njnu.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：DM ; Deltamethrin ; DMSO ; Dimethyl sulphoxide ; es ; Elution solution ; GAPDH ; Glyceraldehyde 3-phosphate dehydrogenase ; PCR ; Polymerase chain reaction ; qPCR ; Quantitative real-time PCR ; dsRNA ; Double-stranded RNA ; RNAi ; RNA interference ; CCK-8 ; Cell Counting Kit-8
- 刊名：Gene
- 出版年：2016
- 出版时间：10 May 2016
- 年：2016
- 卷：582
- 期：1
- 页码：85-93
- 全文大小：727 K
文摘
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448 differentially expressed genes were identified by cDNA microarray analysis after DM treatment.
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Functional classification of genes involved in the DM stress response by pathway and gene ontology analysis.
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The full-length coding sequence of Prosbeta2 was cloned in this study.
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Overexpression of Prosbeta2 enhanced Drosophila cells DM detoxification.
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This study will provide new ideas and targets for studying the molecular mechanisms of insect resistance.