A new, rapid and alternative protocol for fabrication of µPAD have been described.
TEOS was a silylation agent for the filter paper in microwave irradiation.
Hydrophilic channels were successfully generated by ink-jet printing technique.
The limit of detection of developed µPADs was measured 1 unit ml−1 urease enzyme within 3 min.
This new combination allows providing simple-to-use and cost-effective µPADs.