Diclofop-methyl affects microbial rhizosphere community and induces systemic acquired resistance in rice

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• 作者：Si Chen¹ ; ^{cslock@126.com} ; Xingxing Li¹ ; Michel Lavoie² ; Yujian Jin³ ; Jiahui Xu¹ ; Zhengwei Fu³ ; Haifeng Qian⁴ ; ^{hfqian@zjut.edu.cn}
• 关键词：Diclofop-methyl ; Rhizosphere microorganisms ; Organic acids ; Systemic acquired resistance ; Rice
• 刊名：Journal of Environmental Sciences
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：51
• 期：Complete
• 页码：352-360
• 全文大小：860 K
• 卷排序：51

文摘

Diclofop-methyl (DM), a widely used herbicide in food crops, may partly contaminate the soil surface of natural ecosystems in agricultural area and exert toxic effects at low dose to nontarget plants. Even though rhizosphere microorganisms strongly interact with root cells, little is known regarding their potential modulating effect on herbicide toxicity in plants. Here we exposed rice seedlings (Xiushui 63) to 100 μg/L DM for 2 to 8 days and studied the effects of DM on rice rhizosphere microorganisms, rice systemic acquired resistance (SAR) and rice-microorganisms interactions. The results of metagenomic 16S rDNA Illumina tags show that DM increases bacterial biomass and affects their community structure in the rice rhizosphere. After DM treatment, the relative abundance of the bacterium genera Massilia and Anderseniella increased the most relative to the control. In parallel, malate and oxalate exudation by rice roots increased, potentially acting as a carbon source for several rhizosphere bacteria. Transcriptomic analyses suggest that DM induced SAR in rice seedlings through the salicylic acid (but not the jasmonic acid) signal pathway. This response to DM stress conferred resistance to infection by a pathogenic bacterium, but was not influenced by the presence of bacteria in the rhizosphere since SAR transcripts did not change significantly in xenic and axenic plant roots exposed to DM. The present study provides new insights on the response of rice and its associated microorganisms to DM stress.