Ultrasound-Potentiated Salicylic Acid¨CInduced Physiological Effects and Production of Taxol in Hazelnut (Corylus Avellana L.) Cell Culture

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• 作者：Ayatollah Rezaei^&lowast ; ; Faezeh Ghanati^&lowast ; ;   ; ^{ghangia@modares.ac.ir} ; Mehrdad Behmanesh^&dagger ; ; Manijhe Mokhtari-Dizaji^&Dagger ;
• 关键词：Corylus avellana ; Elicitation ; Hazelnut ; Secondary metabolites ; Ultrasound ; Taxol
• 刊名：Ultrasound in Medicine and Biology
• 出版年：2011
• 出版时间：November 2011
• 年：2011
• 卷：37
• 期：11
• 页码：1938-1947
• 全文大小：649 K

文摘

Effects of ultrasound (US), salicylic acid (SA) and their combined use on the growth and secondary metabolite production of suspension-cultured Corylus avellana cells were investigated. The cultures were treated with US (40 kHz) for short periods of time (2, 3, 5 and 10 min) and SA (25 and 50 mg L^?). Results showed that although phenolic content of the cells was significantly increased under exposure to treatments, flavonoids content significantly decreased. Taxol biosynthesis was improved by all treatments. US exposure increased the extracellular, cell-associated and total taxol yield three-, 1.6-, and two-fold compared with that of the control, respectively. SA at all levels was more effective than US in stimulating cell-associated and total taxol production. Combined treatment of US and SA at 50 mg L^? resulted in the most improvement in total taxol production, which was about seven times higher than that of the US, three times higher than that of the SA and 14 times higher than that of the?control. The results suggest a synergism between US and SA in enhancing taxol production by hazelnut cells.