Atrazine effect on specific cytosol and nuclear receptors in rat ventral prostate

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• 作者：Jakominic ; M. ; &Scaron ; imic ; B. ; Kniewald ; Z. ; Kniewald ; J.
• 刊名：Toxicology Letters
• 出版年：1998
• 出版时间：July, 1998
• 年：1998
• 卷：95
• 期：Supplement 1
• 页码：148
• 全文大小：111 K

文摘

The androgen ³H-5α-dihydrotestosterone (³H-DHT) has been used to characterize specific cytosol receptors (CR) and nuclear receptors (NR) in rat ventral prostate under atrazine influence. The study of atrazine-blocking effects on ³H-DHT-CR complex in rat ventral prostate was performed by using sucrose density gradient ultracentrifugation. The formation was observed at 12S. In vitro addition of 0.7 μmoles of atrazine inhibited the 3H-DHT-CR complex formation by 37 % respectively. Characterization of NR in rat ventral prostate under in vitro addition of 0.7 μmoles of atrazine and in vivo i.p. treatment with 60 and 120 mg atrazine per kg bw./10 days daily was performed by 12 % SDS-PAGE and by Scatchard analysis. The ³H-DHT-NR complex from rat prostate analysed by SDS-PAGE was detected at Mr 67 kD. Binding of ³H-DHT to NR in rat prostate was blocked for 41 % in the presence of 0.7 μmoles of atrazine. The number of binding sites on NR molecules evaluated by Scatchard analysis showed the decrease of ³H-DHT-NR complex formation in the presence of 0.7 μmoles of atrazine, while K_d value was without change 10^−12 M.