A histidine in the β-CASP domain of Artemis is critical for its full in vitro
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- 作者：Jean-Pierre de Villartay ; Noriko Shimazaki ; Jean-Baptiste Charbonnier ; Alain Fischer ; Jean-Paul Mornon ; Michael R. Lieber ; Isabelle Callebaut
- 关键词：Artemis ; HCA ; Metallo-β ; -lactamase ; NHEJ ; SCID
- 刊名：DNA Repair
- 出版年：2009
- 出版时间：1 February 2009
- 年：2009
- 卷：8
- 期：2
- 页码：202-208
- 全文大小：1144 K
文摘
Artemis is a key factor of the nonhomologous end-joining (NHEJ) pathway, which is critical for DNA double-strand break (DSB) repair in eukaryotic cells. It belongs to the β-CASP family of nucleases, forming a distinct group within the metallo-β-lactamase superfamily. Proteins of this group are specific for nucleic acids and contain an original domain, the β-CASP domain, which serves as a cap covering the active site displayed by the metallo-β-lactamase domain.Here, we have identified in the highly divergent sequences of the β-CASP domains from DNA-specific nucleases two conserved residues (Artemis E213 and H254), which are not present in RNA-specific enzymes, and shown that H254 plays a key role in the Artemis function, as it is critical for its full activity in vitro. Moreover, inherited mutation of H254 results in radiosensitive severe combined immune deficiency (RS-SCID) in humans. This residue might play a key role in specificity towards DNA, if not directly in zinc binding.