Notch signaling regulates tubular morphogenesis during repair from biliary damage in mice

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• 作者：Romina Fiorotto¹ ; Aileen Raizner¹ ; Carola M. Morell² ; Barbara Torsello³ ; Roberto Scirpo² ; Luca Fabris⁴ ; Carlo Spirli¹ ; Mario Strazzabosco¹ ; ² ; ^{Mario.strazzabosco@yale.edu}
• 关键词：DDC ; 3 ; 5-diethoxycarbonyl-1 ; 4-dihydrocollidine ; ANIT ; alpha-naphthyl-isothiocyanate ; RPB-Jk ; recombination signal binding protein for immunoglobulin kappa J region ; K19 ; cytokeratin 19 ; SOX-9 ; SRY (sex determining region Y)-box 9 ; BMOL ; bipotential mouse
• 刊名：Journal of Hepatology
• 出版年：2013
• 出版时间：July, 2013
• 年：2013
• 卷：59
• 期：1
• 页码：124-130
• 全文大小：1403 K

文摘

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Background & Aims

Repair from biliary damages requires the biliary specification of hepatic progenitor cells and the remodeling of ductular reactive structures into branching biliary tubules. We hypothesized that the morphogenetic role of Notch signaling is maintained during the repair process and have addressed this hypothesis using pharmacologic and genetic models of defective Notch signaling.

Methods

Treatment with DDC (3,5-diethoxycarbonyl-1,4-dihydrocollidine) or ANIT (alpha-naphthyl-isothiocyanate) was used to induce biliary damage in wild type mice and in mice with a liver specific defect in the Notch-2 receptor (Notch-2-cKO) or in RPB-Jk. Hepatic progenitor cells, ductular reaction, and mature ductules were quantified using K19 and SOX-9.

Results

In DDC treated wild type mice, pharmacologic Notch inhibition with dibenzazepine decreased the number of both ductular reaction and hepatic progenitor cells. Notch-2-cKO mice treated with DDC or ANIT accumulated hepatic progenitor cells that failed to progress into mature ducts. In RBP-Jk-cKO mice, mature ducts and hepatic progenitor cells were both significantly reduced with respect to similarly treated wild type mice. The mouse progenitor cell line BMOL cultured on matrigel, formed a tubular network allowing the study of tubule formation in vitro; ¦Ã-secretase inhibitor treatment and siRNAs silencing of Notch-1, Notch-2 or Jagged-1 significantly reduced both the length and number of tubular branches.

Conclusions

These data demonstrate that Notch signaling plays an essential role in biliary repair. Lack of Notch-2 prevents biliary tubule formation, both in vivo and in vitro. Lack of RBP-Jk inhibits the generation of biliary-committed precursors and tubule formation.