Evaluation and validation of reference genes for qPCR analysis to study climate change-induced stresses in Sinularia cf. cruciata (Octocorallia: Alcyonidae)
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- 作者：Gaurav G. Shimpia ; Sergio Vargasa ; Gert Wö ; rheidea ; b ; c ; woerheide@lmu.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：Reference gene ; qPCR ; Octocoral ; Abiotic stress ; Climate change ; Gene expression
- 刊名：Journal of Experimental Marine Biology and Ecology
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：483
- 期：Complete
- 页码：42-52
- 全文大小：1100 K
文摘
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Seven housekeeping genes were tested for expression stability during two abiotic stresses.
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Thermal and pH stress-specific sets of HKGs are proposed as RGs for qPCR in Sinularia.
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ACTB, RPL12, SRP54 and TUBB genes were found most stable in a stress-dependent manner.
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TUBA and EF1A were found unsuitable for use as reference genes.
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Stress-dependent differential expression of HSP70 gene was detected.