Uptake and photo-toxicity of Foscan®, Foslip® and Fospeg® in multicellular tumor spheroids
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- 作者：Elisa Gaioa ; elisa.gaio@studenti.unipd.it" class="auth_mail" title="E-mail the corresponding author ; Dietrich Scheglmannb ; Dietrich.Scheglmann@biolitec.com" class="auth_mail" title="E-mail the corresponding author ; Elena Reddia ; elena.reddi@unipd.it" class="auth_mail" title="E-mail the corresponding author ; Francesca Moreta ; francesca.moret@unipd.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：PDT ; photodynamic therapy ; PS ; photosensitizer ; m-THPC ; meta-tetra(hydroxyphenyl)chlorin ; ROS ; Reactive Oxygen Species ; EPR ; Enhanced Permeability and Retention effect ; PEG ; polyethylene glycol ; CAM ; chick chorioallantoic membrane ; DMEM ; Dulbecco's Modified Eagle Medium ; FBS ; Foetal Bovine Serum ; MTS ; 3-(4 ; 5-dimethyltiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium ; PBS ; Phosphate Buffered Saline ; HBSS ; Hank's Balanced Salt Solution ; SEM ; Scanning Electron Microscopy ; TEM
- 刊名：Journal of Photochemistry and Photobiology B: Biology
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：161
- 期：Complete
- 页码：244-252
- 全文大小：1156 K
文摘
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PDT with Foscan®, Foslip® and Fospeg® was studied in spheroids of HeLa cells.
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Contrary to Foscan®, Fospeg® and Foslip® are not cytotoxic in the dark.
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m-THPC formulations accumulate only in the outer rim of multicellular spheroids.
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Fospeg®- and Foslip®-PDT induce more severe spheroid disruption than Foscan®-PDT
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PDT-induced cell death occurred mainly via apoptosis with all m-THPC formulations