Specific expression and function of inositol 1,4,5-trisphosphate 3-kinase C (ITPKC) in wild type and knock-out mice

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• 作者：Ariane Scoumanne^a ; ¹ ; Patricia Molina-Ortiz^a ; Daniel Monteyne^b ; David Perez-Morga^b ; ^c ; Christophe Erneux^d ; Sté ; phane Schurmans^a ; ^{sschurmans@ulg.ac.be" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Inositol 1 ; 3 ; 4 ; 5-tetrakisphosphate ; Inositol 1 ; 4 ; 5-trisphosphate 3-kinase ; Itpkc ; Tissue expression ; Mouse
• 刊名：Advances in Biological Regulation
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：62
• 期：Complete
• 页码：1-10
• 全文大小：2030 K

文摘

Inositol 1,4,5-trisphosphate 3-kinase C (ITPKC) is the last identified member of the inositol 1,4,5-trisphosphate 3-kinases family which phosphorylates inositol 1,4,5-trisphosphate into inositol 1,3,4,5-tetrakisphosphate. Although expression and function of the two other family members ITPKA and ITPKB are rather well characterized, similar information is lacking for ITPKC. Here, we first defined the expression of Itpkc mRNA and protein in mouse tissues and cells using in situ hybridization and new antibodies. Surprisingly, we found that cells positive for ITPKC in the studied tissues express either a multicilium (tracheal and bronchial epithelia, brain ependymal cells), microvilli forming a brush border (small and large intestine, and kidney proximal tubule cells) or a flagellum (spermatozoa), suggesting a role for ITPKC either in the development or the function of these specialized cellular structures. Given this surprising expression, we then analyzed ITPKC function in multiciliated tracheal epithelial cells and sperm cells using our Itpkc knock-out mouse model. Unfortunately, no significant difference was observed between control and mutant mice for any of the parameters tested, leaving the exact in vivo function of this third Ins(1,4,5)P3 3-kinase still open.