Mechanism of allele specific assembly and disruption of master regulator transcription factor complexes of NF-KBp50, NF-KBp65 and HIF1a on a non-coding FAS SNP
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- 作者：Chidiebere U. Awaha ; b ; Stephanie Tamma ; Silke Hedtfelda ; Doris Steinemannc ; Burkhard Tü ; mmlera ; d ; Georgios Tsiavaliarise ; Frauke Stankea ; d ; Mekus.Frauke@mh-hannover.de" class="auth_mail" title="E-mail the corresponding author
- 关键词：NF-KBp50 ; NF-KBp65 ; HIF1a ; Higher order multimeric TF complexes ; Allele-specific TF binding ; TF-binding affinity ; Non-coding SNP ; Cystic fibrosis
- 刊名：Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：1859
- 期：11
- 页码：1411-1428
- 全文大小：2295 K
文摘
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Allele-specific TF complex assembly and disruption on the non-coding variant is based on binding affinity and dissociation rate.
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0010">NF-KBp50, p65 and HIF1a preferentially bind the C-allele of rs7901656 in FAS.
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0015">NF-KBp50, p65 and HIF1a form higher order heteromultimeric complexes with synergism in binding their motifs around rs7901656.
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p65 and HIF1a binding are considerably influenced by flanking sequences around their motifs while NF-KBp50 is not.
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TF disruption on rs7901656 is associated with cystic fibrosis disease outcome.