Molecular analyses to design primers of influenza A virus are proposed.
Four multiplex RT-PCR assays to detect EIVs were developed.
The mRT-PCR was evaluated with forty-six influenza A viruses from different hosts.
Few cross-reactivity with other animal influenza viruses was observed in the tests.
The mRT-PCR is expected to have a wide range of applications in monitoring and diagnosing EIVs.