A DPO-based mPCR assay for four pathogenic Vibrio species in seafood was developed.
The DPO-based mPCR assay allowed a wide annealing temperature range.
The DPO-based mPCR assay was more specific than conventional PCR assay.
The assay has been validated in food, clinical samples and foodborne outbreaks.
The assay is reliable tool for detecting the four Vibrio species in lab diagnosis.