Effects of ultrasound on the structure and function of tumor necrosis factor–α

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• 作者：Tian ; Zhong-Min ; Wan ; Ming-Xi ; Wang ; Bo ; Wang ; Su-Pin ; Wu ; Xiao-Ming ; Ruan ; Yu-Song
• 关键词：Ultrasound molecular effects ; TNF-α ; High intensity ultrasound ; SDS-PAGE ; MALDI-TOF-MS. Fragmentation ; Stability
• 刊名：Ultrasound in Medicine and Biology
• 出版年：2003
• 出版时间：September, 2003
• 年：2003
• 卷：29
• 期：9
• 页码：1331-1339
• 全文大小：276 K

文摘

The objective of this study was to investigate the effects of ultrasound on the structure and function of human tumor necrosis factor–alpha (TNF-α) and to study whether TNF-alpha underwent a denaturation process and the molecular structure was damaged when it was irradiated by ultrasound. The samples of TNF-alpha were dissolved in aqueous solution and filled into polystyrene tubes. High intensity ultrasound processor (20 kHz frequency, burst mode, 0.5 duty factor, 100–500 W total electrical power, 0–20 min total treatment time) was used during the treatment. The biologic activity of TNF-alpha was determined by its toxic activity towards TNF-alpha sensitive cell line L₉₂₉ in the presence of actinomycin D. The methods of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) were used to detect the integrity of TNF-alpha molecule after it was irradiated by ultrasound. The results showed TNF-alpha could keep its biological activity, instead of undergoing a denaturation process, when it is irradiated by ultrasound in the aqueous solution; at the same time, the aggregates of TNF-alpha formed by the recombinant DNA E. coli could be dissociated through the molecular vibration induced by ultrasound energy. The biologic activity of TNF-alpha was not reduced, but small quantities of TNF-alpha molecular structure were damaged during the process of sonication. These features of TNF-α molecule irradiated by ultrasound probably gave TNF-α the advantage in being used in the drug microencapsulation and provided a new drugs formulation for tumor therapy. (mxwan@mail.xjtu.edu.cn)