Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense

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• 作者：Song Yee Kim ; Young Ae Kang ; Il Kwon Bae ; Jae-Joon Yim ; Moo Suk Park ; Young Sam Kim ; Se Kyu Kim ; Joon Chang ; Seok Hoon Jeong
• 关键词：Mycobacterium abscessus ; Mycobacterium massiliense ; Multilocus sequence analysis ; rpoB ; Multilocus sequence typing
• 刊名：Diagnostic Microbiology and Infectious Disease
• 出版年：2013
• 出版时间：October, 2013
• 年：2013
• 卷：77
• 期：2
• 页码：143-149
• 全文大小：782 K

文摘

This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections.