Exogenous apelin changes alpha and beta myosin heavy chain mRNA expression and improves cardiac function in PTU-induced hypothyroid rats

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• 作者：Farzaneh Faraji Shahrivar^a ; ^b ; Mohammad Badavi^a ; ^{badavim@yahoo.com" class="auth_mail" title="E-mail the corresponding author} ; Mahin Dianat^a ; Ali Mard^c ; Akram Ahangarpour^a ; Alireza Samarbaf-zadeh^d
• 关键词：α-MHC ; α myosin heavy chain ; β-MHC ; β myosin heavy chain ; T4 ; l-Thyroxin ; PTU ; 6-propyl-2-thiouracil ; TSH ; thyroid stimulated hormone ; SVR ; systemic vascular resistance ; APJ receptor ; APJ gene symbol of apelin receptor ; HR ; heart rate ;   ; +  ; dp/dt max ; the maximal rate of rise in the left ventricular pressure during systole ;   ; &minus ;   ; dp/dt max ; the maximum rate of reduction in pressure during diastole ; LVDP ; development of pressure in the left ventricular (difference between lef
• 刊名：Gene
• 出版年：2016
• 出版时间：20 December 2016
• 年：2016
• 卷：595
• 期：1
• 页码：25-30
• 全文大小：1038 K

文摘

The most important conditions associated with hypothyroidism is the cardiac dysfunction. Apelin is an endogenous ligand, involved in energy storage and metabolism which improves cardiac contractility. This study was done to evaluate the effects of apelin, l-Thyroxin (T4) or a combination of both, on cardiac function and mRNA expression of two contractile proteins, α and β myosin heavy chain (α-MHC and β-MHC), in 6-propyl-2-thiouracil (PTU)-induced hypothyroid rats. Forty male Wistar rats were randomly assigned into five groups: Ctrl (Control), and 4 hypothyroid groups (H, HA, HT, and HAT). The Hypothyroid (H) group received 0.05% PTU in the drinking water for six weeks; the next 3 groups, along with PTU, received apelin (HA, 200 μg/kg/day, ip), T4 (HT, 20 μg/kg/day, gavage), or a combination of both drugs (HAT) for the last 2 weeks (weeks 5 and 6). TSH and T4 were measured using ELISA kit. Isolated hearts of animals were perfused in Langendorff apparatus and left ventricular developed pressure, cardiac contractility, heart rate, rate pressure product and perfusion pressure were assessed using PowerLab ADInstruments. In addition α-MHC and β-MHC mRNA expression were evaluated by RT-PCR method in heart tissue. Apelin alone or accompanied by T4 significantly increased cardiac contractility and performance as compared to hypothyroid group. Apelin also significantly increased the alpha-MHC mRNA expression and in the presence of T4 significantly decreased beta-MHC mRNA expression.

It seems that apelin alone may improve cardiac function in hypothyroid rats via genomic pathways.