Morphological and functional alteration of erythrocyte ghosts and giant unilamellar vesicles caused by Vipera latifi venom

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• 作者：Gayane Kirakosyan ; Maryam Mohamadvarzi ; Lusine Ghulikyan ; Naira Zaqaryan ; Arsen Kishmiryan ; Naira Ayvazyan ; ^{taipan@ysu.am" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Vipera latifi ; Erythrocyte ghosts ; GUVs ; [Na+ ; K+]-ATPase and (Ca2  ; +  ; +  ; Mg2  ; +)-ATPases
• 刊名：Comparative Biochemistry and Physiology, Part C
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：190
• 期：Complete
• 页码：48-53
• 全文大小：992 K

文摘

Snake bites are an endemic public health problem in Iran, both in rural and urban area. Viper venom as a hemolytic biochemical “cocktail” of toxins, primarily cause to the systemic alteration of blood cells. In the sixties and seventies, human erythrocytes were extensively studied, but the mechanical and chemical stresses commonly exerted on red blood cells continue to attract interest of scientists for the study of membrane structure and function. Here, we monitor the effect of m>Vipera latifim> venom on human erythrocytes ghost membranes using phase contrast and fluorescent microscopy and changes in ATPase activity under snake venom influence m>in vitrom>. The ion pumps [Na⁺,K⁺]-ATPase and (Ca^2 + + Mg^2 +)-ATPase plays a pivotal role in the active transport of certain cations and maintenance of intracellular electrolyte homeostasis. We also describe the interaction of m>Vipera latifim> (VL) venom with giant unilamellar vesicles (GUVs) composed of the native phospholipid mixtures visualized by the membrane fluorescence probe, ANS, used to assess the state of membrane and specifically mark the phospholipid domains.