Microarray multiplex assay for the simultaneous detection and discrimination of hepatitis B, hepatitis C, and human immunodeficiency type-1 viruses in human blood samples

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• 作者：Chu Chieh Hsia ; Vladimir E. Chizhikov ; Amy X. Yang ; Angamuthu Selvap ; iyan ; Indira Hewlett ; Robert Duncan ; Raj K. Puri ; Hira L. Nakhasi ; Gerardo G. Kaplan
• 关键词：Microarray technology ; Human immunodeficiency virus type-1 (HIV-1) ; Hepatitis C virus (HCV) ; Hepatitis B virus (HBV) ; Multiplex detection and discrimination ; PCR ; Blood-borne virus
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：2007
• 出版时间：18 May 2007
• 年：2007
• 卷：356
• 期：4
• 页码：1017-1023
• 全文大小：926 K

文摘

Hepatitis B virus (HBV), hepatitis C virus (HCV), and human immunodeficiency virus type-1 (HIV-1) are transfusion-transmitted human pathogens that have a major impact on blood safety and public health worldwide. We developed a microarray multiplex assay for the simultaneous detection and discrimination of these three viruses. The microarray consists of 16 oligonucleotide probes, immobilized on a silylated glass slide. Amplicons from multiplex PCR were labeled with Cy-5 and hybridized to the microarray. The assay detected 1 International Unit (IU), 10 IU, 20 IU of HBV, HCV, and HIV-1, respectively, in a single multiplex reaction. The assay also detected and discriminated the presence of two or three of these viruses in a single sample. Our data represent a proof-of-concept for the possible use of highly sensitive multiplex microarray assay to screen and confirm the presence of these viruses in blood donors and patients.