Characterization of cetuximab Fc/2 dimers by off-line CZE-MS
详细信息 查看全文
- 作者：Yannis-Nicolas Franç ; oisa ; yfrancois@unistra.fr" class="auth_mail" title="E-mail the corresponding author ; Michael Biacchia ; Nassur Saida ; Charly Renarda ; Alain Beckb ; Rabah Gahouala ; c ; Emmanuelle Leize-Wagnera
- 关键词：Capillary electrophoresis-mass spectrometry ; Monoclonal antibody ; Dimers ; NanoElectrospray
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：18 February 2016
- 年：2016
- 卷：908
- 期：Complete
- 页码：168-176
- 全文大小：1411 K
文摘
- •
We confirm the power of off-line CE/MS coupling for the separation of mAb isoforms.
- •
We demonstrate the role of C-terminal lysine truncation in the separation process.
- •
We demonstrate the presence of non-covalent interactions between Fc/2 subunit.
- •
We identify Fc/2 homo- and heterodimers.