Serum albumin as a probe for testing the selectivity of irreversible cysteine protease inhibitors: The case of vinyl sulfones
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- 作者：Luca Regazzoni ; luca.regazzoni@unimi.it" class="auth_mail" title="E-mail the corresponding author ; Simone Colombo ; Angelica Mazzolari ; Giulio Vistoli ; Marina Carini
- 关键词：CP ; cysteine protease ; PVS ; phenyl vinyl sulfone ; PPS ; 1-penten-1-yl phenyl sulfone ; MSS ; mesityl styryl sulfone ; CTS ; 1-cyclohexenyl p-tolyl sulfone ; CH3CN ; acetonitrile ; H2O ; water ; HCOOH ; formic acid ; ESI ; electrospray ionization ; NSI ; nanoelectrospray ionization ; AGC ; automatic gain control ; CID ; collison induced dissociation ; Cys ; cysteine ; Lys ; lysine ; His ; histidine ; HSA ; human serum albumin ; MS ; mass spectrometry ; MS/MS ; tandem mass spectrometry ; LC ; liquid chromatography ; SIC ; single io
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：30 May 2016
- 年：2016
- 卷：124
- 期：Complete
- 页码：294-302
- 全文大小：1412 K
文摘
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Vinyl sulfones covalently react with albumin.
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Vinyl sulfones are not selective alkylants of cysteine residues.
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Albumin is a good probe for testing the selectivity of covalent inhibitors of cysteine proteases.