Rapid DNA transformation in Salmonella Typhimurium by the hydrogel exposure method
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- 作者：Hamouda Elabeda ; elabedhamouda@yahoo.fr" class="auth_mail" title="E-mail the corresponding author ; Rim Hamzab ; Amina Bakhroufa ; Kamel Gaddourb
- 关键词：Salmonella ; Transformation ; Sepiolite
- 刊名：Journal of Microbiological Methods
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：126
- 期：Complete
- 页码：67-71
- 全文大小：460 K
文摘
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A protocol of Salmonella transformation using sepiolite nanofibers was optimized.
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The tested variables are: amount of sepiolite, number of cells and spreading time.
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Using the general protocol, the efficiency was about 5 × 105 per 1 μg of plasmid.
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The method is simple, rapid, and inexpensive and does not require competent cell preparation.