pH sensitive dexamethasone encapsulated laponite nanoplatelets: Release mechanism and cytotoxicity
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- 作者：M. Roozbahani ; M. Kharaziha ; ma.kharaziha@gmail.com ; R. Emadi
- 关键词：Dexamethasone ; Laponite nanoplates ; Cytotoxicity ; Drug release
- 刊名：International Journal of Pharmaceutics
- 出版年：2017
- 出版时间：25 February 2017
- 年：2017
- 卷：518
- 期：1-2
- 页码：312-319
- 全文大小：2331 K
- 卷排序：518
文摘
The purpose of this study was to develop an efficient strategy to use laponite (LAP) nanoplates as a platform for the efficient release of anionic dexamethasone (DEX). Results revealed that DEX was encapsulated into the interlayer space of LAP nanodisks through an intercalation process with a high loading efficiency of 95.10 ± 0.80%. X-Ray diffraction (XRD) patterns and Fourier transform infrared (FTIR) spectra of the hybrid LAP/DEX nanoplates (LD-NPs) indicated that DEX molecules could successfully adsorb into the LAP nanoplates depending on the pH value. Moreover, in vitro drug release study showed that the release of DEX from LD-NPs was pH-dependent, and DEX released at a faster rate at acidic pH (pH = 5.4) than physiological one. Importantly, the results of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay confirmed that the released DEX from LD-NPs not only did not show cytotoxic effect but also improved the viability of MG63 cells compared to LAP-free samples (DEX enriched medium). Our work indicated that LAP nanoplates could be a promising candidate for release of anionic DEX in the controlled manner depending on the pH environment. Moreover, the merits of LD-NPs such as good cytocompatibility, excellent physiological stability and sustained pH-responsive release properties, make them a promising platform for the delivery of other therapeutic agents beyond DEX.