Electrochemical and spectroscopic investigations of carboxylic acid ligand and its triorganotin complexes for their binding with ds.DNA: In vitro biological studies
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- 作者：Nasima Arshad ; Shahid Iqbal Farooqi ; Moazzam H. Bhatti ; Samreen Saleem ; Bushra Mirza
- 关键词：Triorganotin(IV) carboxylates ; UV&ndash ; visible/fluorescence spectroscopy ; Cyclic voltammerty ; Intercalation ; Binding constant/site size ; Bioactivities
- 刊名：Journal of Photochemistry and Photobiology B: Biology
- 出版年：2013
- 出版时间：5 August, 2013
- 年：2013
- 卷：125
- 期：Complete
- 页码：70-82
- 全文大小：2369 K
文摘
A carboxylic acid ligand, (Z)-4-(4-acetylphenylamino)-4-oxobut-2-enoic acid (APA-1), and its triphenyl-(APA-2) and tributyl-tin(IV) (APA-3) compounds have been synthesized and investigated for their binding with ds.DNA using UV-visible spectroscopy, fluorescence spectroscopy, cyclic voltammetry, and viscosity measurements under physiological conditions of pH and temperature. The experimental results from all techniques i.e. binding constant (Kb), binding site size (n) and free energy change (¦¤G) were in good agreement and inferred spontaneous compound-DNA complexes formation via intercalation. Among all the compounds APA-3 showed comparatively greater binding at pH 4.7 as evident from its greater Kb values {APA-3: Kb: 5.63 ¡Á 104 M?1 (UV); 7.94 ¡Á 104 M?1 (fluorescence); 9.91 ¡Á 104 M?1 (CV)}. Electrochemical processes of compounds before and after the addition of DNA were found diffusion controlled. Among all compounds, APA-3 exhibited best antitumor activity.