Sample preparation of serum to allow capillary electrophoresis analysis of prostate specific antigen isoforms

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• 作者：Noemi Farina-Gomezp>ap> ; Silvia Barrabesp>bp> ; Jorge E. Gomez-Lopezp>ap>p> ; p>p>cp>p> ; p>p>1p> ; Monica Gonzalezp>ap> ; Angel Puertap>ap> ; Diana Navarro-Calderonp>ap> ; Eduardo Albers-Acostap>dp> ; Carlos Olivierp>dp> ; Jose C. Diez-Masap>ap> ; Rosa Peracaulap>bp>p> ; p>p>rosa.peracaula@udg.edup> ; Mercedes de Frutosp>ap>p> ; p>p>mfrutos@iqog.csic.esp>
• 关键词：ACT ; alpha-1 antichymotrypsin ; 2-DE ; two-dimensional electrophoresis ; fPSA ; free PSA ; HRP ; horseradish peroxidase ; IAC ; immunoaffinity chromatography ; PSA ; prostate specific antigen ; tPSA ; total PSA ; Tris ; Tris (hydroxymethyl) aminomethane ; WB ; Western blot
• 刊名：Journal of Pharmaceutical and Biomedical Analysis
• 出版年：2017
• 出版时间：5 February 2017
• 年：2017
• 卷：134
• 期：Complete
• 页码：220-227
• 全文大小：1306 K
• 卷排序：134

文摘

A method to prepare serum samples to make CE analysis of PSA possible is shown. The effect of sample preparation on PSA is followed by SDS-PAGE, CD, CZE and 2-DE. Ethanolamine and chromatographic immunopurification do not markedly alter PSA. CZE profiles of PSA vary in cancer-patient serum and healthy-person seminal plasma.