The mature sequence of GH3 Aspergillus niger 尾-xylosidase (AnBX) was cloned and expressed.
The expression level was increased to 5.7 g/l by codon optimization and fermentation.
AnBX could be purified through a single-step Phenyl Sepharose chromatography.
AnBX exhibited an exo-type, 尾-retaining activity, and act synergistically with xylanase.
AnBX may be useful for degradation of lignocellulosic biomass.