Cloning, expression and characterization of 尾-xylosidase from Aspergillus niger ASKU28
详细信息 查看全文
- 作者：Khuanjarat Choengpanyaa ; Siriphan Arthornthurasukb ; Pakorn Wattana-amornc ; Wan-Ting Huangd ; Wandee Plengmuankhaeb ; Yaw-Kuen Lid ; Prachumporn T. Kongsaereea ; b ; e ; fscippt@ku.ac.th" class="auth_mail" title="E-mail the corresponding author
- 关键词：AnBX ; Aspergillus niger ASKU28 尾-xylosidase ; 3 ; 4-DNP ; 3 ; 4-dinitrophenol ; 3 ; 4-DNP-Xyl ; 3 ; 4-dinitrophenyl-尾-d-xyloside ; DNS ; 3 ; 5-dinitrosalicylic acid ; GH ; glycoside hydrolase family ; 伪-MF ; Saccharomyces cerevisiae 伪-mating factor secretion signal ; pNP ; para-nitrophenol ; pNP-Ara ; para-nitrophenyl-伪-l-arabinoside ; pNP-Glc ; para-nitrophenyl-尾-d-glucoside ; pNP-Xyl ; para-nitrophenyl-尾-d-xyloside
- 刊名：Protein Expression and Purification
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：115
- 期：Complete
- 页码：132-140
- 全文大小：1160 K
文摘
- •
The mature sequence of GH3 Aspergillus niger 尾-xylosidase (AnBX) was cloned and expressed.
- •
The expression level was increased to 5.7 g/l by codon optimization and fermentation.
- •
AnBX could be purified through a single-step Phenyl Sepharose chromatography.
- •
AnBX exhibited an exo-type, 尾-retaining activity, and act synergistically with xylanase.
- •
AnBX may be useful for degradation of lignocellulosic biomass.