Cloning and expression analysis of nonspecific cytotoxic cell receptor 1 (Ls-NCCRP1) from red snapper (Lutjanus sanguineus)
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- 作者：Jia Cai ; Shina Wei ; Bei Wang ; Yucong Huang ; Jufen Tang ; Yishan Lu ; Zaohe Wu ; Jichang Jian
- 关键词：Red snapper ; Innate immune ; NCCRP-1 ; Vibrio alginolyticus
- 刊名：Marine Genomics
- 出版年：2013
- 出版时间：September, 2013
- 年：2013
- 卷：11
- 期：Complete
- 页码：39-44
- 全文大小：1129 K
文摘
It is well known that nonspecific cytotoxic cells (NCCs) are kinds of natural killer cell mediated innate immune responses in teleosts. The nonspecific cytotoxic cell receptor protein 1 (NCCRP-1) is an important cell surface protein on NCC, which serves crucial functions in target cell recognition and cytotoxicity activation. In the present study, a nonspecific cytotoxic cell receptor protein NCCRP-1 (Ls-NCCRP1) was cloned from red snapper, Lutjanus sanguineus. The Ls-NCCRP1 cDNA is composed of 986 bp with a 43 bp of 5¡ä-UTR, 702 bp open reading frame (ORF) and 241 bp 3¡ä-UTR, encoding a polypeptide of 233 amino acids (GenBank accession no: ). Phylogenetic analysis revealed that Ls-NCCRP1 showed highest similarity to sea bream NCCRP-1. Quantitative real-time PCR (qRT-PCR) analysis showed that Ls-NCCRP1 had relatively high expression level in the head kidney, spleen and liver. After Vibrio alginolyticus infection, transcripts of Ls-NCCRP1 increased and reached its peak at 4 h p.i. These results indicated that Ls-NCCRP1 may play an important role in innate immune response to bacteria.