Efficacy of an antiviral compound to inhibit replication of multiple pestivirus species

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• 作者：Benjamin W. Newcomer^a ; ^{bwn0001@auburn.edu} ; M. Shonda Marley^b ; ^{edensms@auburn.edu} ; Julia F. Ridpath^c ; ^{julia.ridpath@ars.usda.gov} ; John D. Neill^c ; ^{john.neill@ars.usda.gov} ; David W. Boykin^d ; ^{dboykin@gsu.edu} ; Arvind Kumar^d ; ^{rasaayan@yahoo.com} ; M. Daniel Givens^b ; ^{givenmd@auburn.edu}
• 关键词：Pestivirus ; Antiviral ; Bovine viral diarrhea virus ; Border disease virus
• 刊名：Antiviral Research
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：96
• 期：2
• 页码：127-129
• 全文大小：158 K

文摘

Pestiviruses are economically important pathogens of livestock. An aromatic cationic compound (DB772) has previously been shown to inhibit bovine viral diarrhea virus (BVDV) type 1 in vitro at concentrations lacking cytotoxic side effects. The aim of this study was to determine the scope of antiviral activity of DB772 among diverse pestiviruses. Isolates of BVDV 2, border disease virus (BDV), HoBi virus, pronghorn virus and Bungowannah virus were tested for in vitro susceptibility to DB772 by incubating infected cells in medium containing 0, 0.006, 0.01, 0.02, 0.05, 0.1, 0.2, 0.39, 0.78, 1.56, 3.125, 6.25, 12.5 or 25 ¦ÌM DB772. The samples were assayed for the presence of virus by virus isolation and titration (BDV and BVDV 2) or PCR (HoBi, pronghorn and Bungowannah viruses). Cytotoxicity of the compound was assayed for each cell type. Complete inhibition of BVDV 2, BDV, and Pronghorn virus was detected when DB772 was included in the culture media at concentrations of 0.20 ¦ÌM and higher. In two of three tests, a concentration of 0.05 ¦ÌM DB772 was sufficient to completely inhibit HoBi virus replication. Bungowannah virus was completely inhibited at a concentration of 0.01 ¦ÌM DB772. Thus, DB772 effectively inhibits all pestiviruses studied at concentrations >0.20 ¦ÌM. As cytotoxicity is not evident at these concentrations, this antiviral compound potentially represents an effective preventative or therapeutic for diverse pestiviruses.