Protective effect of Raphanus sativus on D-galactosamine induced nephrotoxicity in rats

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• 作者：Magesh Salai Bojan^a ; ^{kumar.magesh06@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; Rashmi Rajappa^a ; Dhana Rangesh Kumar Vijayakumar^b ; Jayathi Gopalan^c
• 关键词：Raphanus sativus ; Galactosamine ; Antioxidants ; Free radicals ; Kidney damage
• 刊名：Nutrition Clinique et Métabolisme
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：30
• 期：1
• 页码：22-28
• 全文大小：329 K

文摘

The aim of the present study was to evaluate nephroprotective effect of Raphanus sativus ethanolic extract (RSEt) on tissue defense system in galactosamine (GalN) induced renal damage in rats. GalN was administered intraperitoneally at a dose of 400 mg/kg/b.w for three alternate days and the renal toxicity was manifested by a significant (P < 0.05) increase in the levels of renal markers such as urea, creatinine and uric acid. This was found to be associated with decreased activities of renal antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST), glutathione peroxidase (GPx) and glutathione reductase (GR) and depletion of renal reduced glutathione (GSH), vitamin C and vitamin E. Administration of the RSEt (850 mg/kg/body weight, oral) for 15 days to rats reduced the levels of renal markers and significantly increased the level of antioxidants. The activities of gamma glutamyl transpeptidase (GGT) and thiobarbituric acid reactive substances (TBARS) were also decreased in the kidney of RSEt treated group. Renal histology examination confirmed the damage to the kidney as it reveals severe necrosis of the proximal renal tubules with haemorrhage which was ameliorated by the treatment with RSEt. These results suggest that the R. sativus has protective effects on GalN-mediated nephrotoxicity and this may be related to the action of the antioxidant content of the extract.