Cilostazol attenuates gentamicin-induced nephrotoxicity in rats

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• 作者：Ahmed A. Abdelsameea^a ; Ahmed M. Mohamed^a ; Mona G. Amer^b ; ^{mona_amer@rocketmail.com" class="auth_mail" title="E-mail the corresponding author} ; Shahera M. Attia^c
• 关键词：CAT ; catalase ; DCT ; distal convoluted tubule ; GSH ; Glutathione ; H&E ; hematoxylin and eosin ; MDA ; malondialdehyde ; PCT ; proximal convoluted tubules ; SOD ; superoxide dismutase
• 刊名：Experimental and Toxicologic Pathology
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：68
• 期：4
• 页码：247-253
• 全文大小：2907 K

文摘

Gentamycin is a widely used antibiotic. The nephrotoxic adverse effects of the drug may limit its use. Cilostazol, a phosphodiesterase III inhibitor, was reported to protect from renal oxidative stress.

This work aimed to investigate the possible protective effect of cilostazol on gentamicin-induced nephrotoxicity and the possible underlying mechanisms.

Materials and methods

40 male albino rats were divided into 4 equal groups: (1) Control; (2) Cilostazol, 10 mg/kg, p.o.; (3) Gentamicin, 80 mg/kg, i.p.; (4) Gentamicin 80 mg/kg, i.p. along with cilostazol 10 mg/kg, p.o. All drugs were administered once daily for 8days. On 9th day blood samples were collected for the estimation of creatinine, urea and uric acid in serum. Then the rats were sacrificed and kidneys were removed for light and electron microscope studies. Moreover, reduced glutathione (GSH) and malondialdehyde (MDA) levels as well as catalase (CAT) and superoxide dismutase (SOD) activities were determined in renal tissues.

Results

Gentamicin elevated the serum levels of creatinine, urea and uric acid as well as the MDA level in the renal tissue, while it decreased CAT, SOD activities and GSH levels as well as produced degenerative changes in glomeruli and tubules associated with increased expression of apoptotic markers and decreased expression of anti-apoptotic markers. Administration of cilostazol decreased urea, creatinine, uric acid and MDA levels while increased CAT and SOD activities and GSH levels as well as ameliorated the histopathological changes in relation to gentamicin group.

Conclusion

Cilostazol protected rats from gentamicin-induced nephrotoxicity possibly, in part through its antioxidant and anti-apoptotic activity.