Melatonin can attenuate ciprofloxacin induced nephrotoxicity: Involvement of nitric oxide and TNF-α

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• 作者：Fatemeh Shaki^a ; Sorour Ashari^b ; Nematollah Ahangar^c ; ^{n.ahangar@mazums.ac.ir}
• 关键词：Ciprofloxacin ; Melatonin ; Nephrotoxicity ; Oxidative stress ; NO ; TNF-α
• 刊名：Biomedicine & Pharmacotherapy
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：84
• 期：Complete
• 页码：1172-1178
• 全文大小：1168 K
• 卷排序：84

文摘

Ciprofloxacin is a synthetic broad-spectrum antimicrobial agent of fluoroquinolone family. The aim of our investigation was to evaluate the role of oxidative damage and inflammation in nephrotoxic potential of Ciprofloxacin and protective effects of melatonin against its nephrotoxicity in male Wistar rats.MethodsThe animals were divided into six groups: Control, ciprofloxacin (100 mg/kg/day, i.p), ciprofloxacin with three doses (2.5, 5 and 10 mg/kg/day) of melatonin and a group which received ciprofloxacin (100 mg/kg/day) plus vitamin E (100 mg/kg/day) for 8 consecutive days. 24 h after last injection, the animals were euthanized and kidney tissues were separated. Finally reactive oxygen species, glutathione content, lipid peroxidation, protein carbonyl, nitric oxide and TNF-α were evaluated. Also, pathological examination and measuring of kidney biochemical markers (BUN and Cr) were done.ResultsThe administration of ciprofloxacin for 8 days resulted in significant increase (P < 0.01) in kidney biomarkers (BUN and Cr) and pathological changes. Also, Oxidative stress was evident in ciprofloxacin group by significantly (p < 0.001) increased reactive oxygen species, lipid peroxidation and protein carbonyl level and decreased glutathione content (p < 0.001). Increased in inflammation process was shown by increase in NO and TNF-α (P < 0.001). Administration of melatonin was able to protect against deterioration in nephrotoxic markers and suppressed the increase in oxidative stress and inflammatory markers.ConclusionOur study showed the critical role of oxidative damage and inflammation in ciprofloxacin-induced nephrotoxicity that markedly inhibited by administration of melatonin. So, melatonin can be suggested for prevention of ciprofloxacin-induced nephrotoxicity.