The study was envisaged to explore the adjuvant nNOS inhibition with valproate for combined treatment epilepsy and comorbid depression. Neurochemical changes suggest elevated nitrosative may be responsible for depression associated with epilepsy. Valproate treatment in kindled animals decreased seizure severity, but was found unable to curb elevated nitrosative stress. Adjuvant 7-nitroindazole (selective nNOS inhibitor) with valproate ameliorated both epilepsy and associated depression.