Thymic stromal lymphopoietin is induced by respiratory syncytial virus-infected airway epithelial cells and promotes a type 2 response to infection

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• 作者：Hai-Chon Lee ; PhD^a ; ^&lowast ; ; Mark B. Headley ; PhD^a ; ^b ; ^&lowast ; ; Yueh-Ming Loo ; PhD^b ; Aaron Berlin ; BS^c ; Michael Gale ; PhD^b ; Jason S. Debley ; MD^d ; ^e ; Nicholas W. Lukacs ; PhD^c ; Steven F. Ziegler ; PhD^a ; ^b ; ^{sziegler@benaroyaresearch.org}
• 关键词：TSLP ; RSV ; asthma ; epithelium ; TH2
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2012
• 出版时间：November, 2012
• 年：2012
• 卷：130
• 期：5
• 页码：1187-1196.e5
• 全文大小：1139 K

文摘

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Background

Respiratory viral infection, including respiratory syncytial virus (RSV) and rhinovirus, has been linked to respiratory disease in pediatric patients, including severe acute bronchiolitis and asthma exacerbation.

Objective

The study examined the role of the epithelial-derived cytokine thymic stromal lymphopoietin (TSLP) in the response to RSV infection.

Methods

Infection of human airway epithelial cells was used to examine TSLP induction after RSV infection. Air-liquid interface cultures from healthy children and children with asthma were also tested for TSLP production after infection. Finally, a mouse model was used to directly test the role of TSLP signaling in the response to RSV infection.

Results

Infection of airway epithelial cells with RSV led to the production of TSLP via activation of an innate signaling pathway that involved retinoic acid induced gene I, interferon promoter-stimulating factor 1, and nuclear factor-¦ÊB. Consistent with this observation, airway epithelial cells from asthmatic children a produced significantly greater levels of TSLP after RSV infection than cells from healthy children. In mouse models, RSV-induced TSLP expression was found to be critical for the development of immunopathology.

Conclusion

These findings suggest that RSV can use an innate antiviral signaling pathway to drive a potentially nonproductive immune response and has important implications for the role of TSLP in viral immune responses in general.