Targeted profiling of polar intracellular metabolites using ion-pair-high performance liquid chromatography and -ultra high performance liquid chromatography coupled to tandem mass spectrometry: Applications to serum, urine and tissue extracts
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- 作者：Filippos Michopoulosa ; b ; Filippos.Michopoulos@astrazeneca.com" class="auth_mail ; Nicky Whalleya ; Georgios Theodoridisb ; Ian D. Wilsonc ; Tom P.J. Dunkleya ; Susan E. Critchlowa
- 关键词：Metabolite profiling ; HILIC ; Porous graphitic carbon ; Ion pair chromatography ; Mass spectrometry ; Central carbon metabolism
- 刊名：Journal of Chromatography A
- 出版年：4 July 2014
- 年：2014
- 卷：1349
- 期：Complete
- 页码：60-68
- 全文大小：1357 K
文摘
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Different chromatographic approaches investigated for endogenous metabolite analysis.
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Ion pair chromatography showed the best metabolite coverage.
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40 matrix injections required to “condition” a new chromatographic column.
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Analytical platform applied for urine, plasma and tissue extracts analysis.