Effect of nitrogen-doped graphene quantum dots on the fibrillation of hen egg-white lysozyme

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• 作者：Hua-jin Zeng^a ; Min Miao^b ; Zhe Liu^a ; Ran Yang^b ; ^{yangran@zzu.edu.cn} ; Ling-bo Qu^b
• 关键词：Hen egg-white lysozyme ; Nitrogen-doped graphene quantum dots ; Amyloid fibril ; Inhibition
• 刊名：International Journal of Biological Macromolecules
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：95
• 期：Complete
• 页码：856-861
• 全文大小：3239 K
• 卷排序：95

文摘

In this study, the fibrillation of hen egg-white lysozyme (HEWL) in the absence and presence of different amount of nitrogen-doped graphene quantum dots (N-GQDs) was studied by Thioflavin T (ThT) spectroscopy, Congo red (CR) binding assays, 8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence assay, circular dichroism (CD) and transmission electron microscopy (TEM). The experimental results indicated that not only the fibrillation of HEWL at high temperature (65 °C) and low pH (pH = 2.0) could be inhibited effectively by N-GQDs, but the inhibition of HEWL by N-GQDs followed a dose-dependent manner. The results of this work suggested that the N-GQDs had a great potential for designing new therapeutic agents and were promising for future treatment of amyloid-related diseases.