Differential impairment of vascularization and angiogenesis in bisphosphonate-associated osteonecrosis of the jaw-related mucoperiosteal tissue

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• 作者：Falk Wehrhan MD ; DMD^a ; ^{falk.wehrhan@uk-erlangen.de"" rel=""nofollow} ; Phillip Stockmann MD ; DMD^a ; Emeka Nkenke MD ; DMD ; PhD^a ; Karl A. Schlegel MD ; DMD ; PhD^a ; Arndt Guentsch DMD^b ; Theresia Wehrhan^a ; Friedrich W. Neukam MD ; DMD ; PhD^a ; Kerstin Amann MD^c
• 刊名：Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology
• 出版年：2011
• 出版时间：August 2011
• 年：2011
• 卷：112
• 期：2
• 页码：216-221
• 全文大小：636 K

文摘

Objectives

Impaired vascularization in the etiopathology of aminobisphosphonate-associated osteonecrosis of the jaw (BONJ) is assumed, but evidence is lacking. This immunohistochemical study differentiated vascularization and angiogenesis in BONJ-adjacent mucoperiosteal tissue.

Study design

Twenty BONJ (after zoledronate treatment) and 20 control mucoperiosteal tissue samples were processed with an autostaining-based alkaline phosphatase-antialkaline phosphatase staining kit. Vascularization was assessed by CD31 staining and angiogenesis-related neovessels by CD105 staining. The ratio of stained capillary area to total area of visible field was assessed. Statistics included Bonferroni adjustment.

Results

CD31-stained microvessels were detected in each section and CD105-stained neovessels in each control. BONJ-adjacent mucoperiosteal tissue showed significantly fewer CD105-positive vessels in capillary areas (P < .05) than control samples. CD31-stained capillary area was not significantly reduced in mucoperiosteal BONJ-samples.

Conclusions

Angiogenesis is impaired in BONJ-related mucoperiosteal tissue, but vascularization remains unaffected. Vessel remodeling and neovessel formation is delayed in BONJ, resulting in impaired tissue regeneration of bisphosphonate-exposed oral mucosa.