Failure in activation of the canonical NF-¦ÊB pathway by human T-cell leukemia virus type 1 Tax in non-hematopoietic cell lines

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• 作者：Terumi Mizukoshi ; Hideyuki Komori ; Mariko Mizuguchi ; Hussein Abdelaziz ; Toshifumi Hara ; Masaya Higuchi ; Yuetsu Tanaka ; Yoshiro Ohara ; Noriko Funato ; Masahiro Fujii ; Masataka Nakamura
• 关键词：HTLV-1 ; Tax1 ; NF-¦ÊB ; Canonical pathway ; Transcription ; Hematopoietic cells
• 刊名：Virology
• 出版年：2013
• 出版时间：1 September, 2013
• 年：2013
• 卷：443
• 期：2
• 页码：226-235
• 全文大小：2781 K

文摘

Human T-cell leukemia virus type 1 (HTLV-1) Tax (Tax1) plays crucial roles in leukemogenesis in part through activation of NF-¦ÊB. In this study, we demonstrated that Tax1 activated an NF-¦ÊB binding (gp¦ÊB) site of the gp34/OX40 ligand gene in a cell type-dependent manner. Our examination showed that the gp¦Ê¦¢ site and authentic NF-¦ÊB (Ig¦ÊB) site were activated by Tax1 in hematopoietic cell lines. Non-hematopoietic cell lines including hepatoma and fibroblast cell lines were not permissive to Tax1-mediated activation of the gp¦ÊB site, while the Ig¦ÊB site was activated in those cells in association with binding of RelB. However RelA binding was not observed in the gp¦ÊB and Ig¦ÊB sites. Our results suggest that HTLV-1 Tax1 fails to activate the canonical pathway of NF-¦ÊB in non-hematopoietic cell lines. Cell type-dependent activation of NF-¦ÊB by Tax1 could be associated with pathogenesis by HTLV-1 infection.