Critical phases of viral production processes monitored by capacitance

详细信息    查看全文

• 作者：Emma Petiot^a ; ^b ; ^{emma.petiot@univ-lyon1.fr} ; Sven Ansorge^a ; ^{sven.ansorge@cnrc-nrc.gc.ca} ; Manuel Rosa-Calatrava^b ; ^{manuel.rosa-calatrava@univ-lyon1.frm} ; Amine Kamen^a ; ^c ; ^{amine.kamen@mcgill.ca}
• 关键词：On-line monitoring ; Capacitance probe ; Permittivity ; Characteristic frequency fc ; Virus production kinetics ; Dielectric cell properties ; Membrane capacitance ; Viral infection
• 刊名：Journal of Biotechnology
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：242
• 期：Complete
• 页码：19-29
• 全文大小：2749 K
• 卷排序：242

文摘

Characteristic capacitance signals and cell dielectric properties are highly correlated with viral replication phases. Capacitance allow for on-line monitoring, supervision and control of cell-based viral production processes. The study present production processes with enveloped and lytic viruses, two different cell lines and various production modes.