A Disease-Causing Variant in PCNA Disrupts a Promiscuous Protein Binding Site
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- 作者：Caroline M. Duffy ; Brendan J. Hilbert ; Brian A. Kelch
- 关键词：NER ; nucleotide excision repair ; SILAC ; stable isotope labeling by amino acids in culture ; ITC ; isothermal titration calorimetry ; RER ; ribonucleotide excision repair ; PIP ; PCNA interacting protein ; APIM ; AlkB homolog 2 PCNA interacting motif ; PCNA ; Proliferating cell nuclear antigen
- 刊名：Journal of Molecular Biology
- 出版年：2016
- 出版时间：27 March 2016
- 年：2016
- 卷：428
- 期：6
- 页码：1023-1040
- 全文大小：2436 K
文摘
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Disease mutation causes large deformation of the binding pocket in PCNA.
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Disrupts binding of some, but not all, interaction partners.
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Binding site structure is pliable and accommodates ligands idiosyncratically.
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Loss of interaction with RNase H2B suggests an RER defect in patients.