Evaluation of detection methods for non-O157 Shiga toxin-producing Escherichia coli from food
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- 作者：Bavo Verhaegena ; b ; Inge Van Dammeb ; Marc Heyndrickxa ; c ; Nadine Botteldoornd ; Mohamed Elhadidye ; Karen Verstraetea ; Katelijne Dierickd ; Sarah Denayerd ; Lieven De Zutterb ; Koen De Reua ; Koen.DeReu@ilvo.vlaanderen.be" class="auth_mail" title="E-mail the corresponding author
- 关键词：Shiga toxin-producing Escherichia coli (STEC) ; Detection ; Isolation ; Food ; Acid treatment procedure
- 刊名：International Journal of Food Microbiology
- 出版年：2016
- 出版时间：16 February 2016
- 年：2016
- 卷：219
- 期：Complete
- 页码：64-70
- 全文大小：569 K
文摘
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We examined culture-based detection methods for non-O157 STEC in both artificially and naturally contaminated food.
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Enriched samples with a real-time PCR threshold value above 25 merit an additional acid treatment procedure.
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All isolations should preferably be performed using both a selective and less-selective isolation medium in parallel.