Relationship between F-specific RNA phage genogroups, faecal pollution indicators and human adenoviruses in river water

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• 作者：Leslie Ogorzaly ; Adeline Tissier ; Isabelle Bertr ; Arm ; Maul ; Christophe Gantzer
• 关键词：F-specific RNA phages ; Genotyping ; Real-time RT-PCR ; River water ; Faecal pollution
• 刊名：Water Research
• 出版年：2009
• 出版时间：March 2009
• 年：2009
• 卷：43
• 期：5
• 页码：1257-1264
• 全文大小：346 K

文摘

Recent studies have shown the increasing interest of F-specific RNA phage genotyping to identify major sources of faecal contamination in waters. This study, conducted in a river located in an urbanized watershed with recognized anthropogenic influences, was aimed at evaluating the relevance of direct phage genotyping by real-time RT-PCR. One hundred percent of positive results were obtained with a 5 mL aliquot of river water (n = 31). Phage distribution was modified after cultivation, since the ratio of the two most abundant genogroups (II and I) reached 1.51 log₁₀ by direct RT-PCR-based method versus 0.30 log₁₀ after cultivation (n = 8). For the first time, positive correlations between the concentrations of genogroup II, bacterial indicators and human adenoviruses were observed, which may indicate a human faecal pollution. No correlation between genogroups II and I has been revealed. The concentration of genogroup I was only correlated with water turbidity, suggesting an animal pollution coming from upstream after rainfall events. Among the microbiological parameters studied, only genogroup II/genogroup I ratio shows variations occurring in the major sources of faecal pollution.